The plant defensin NaD1 introduces membrane disorder through a specific interaction with the lipid, phosphatidylinositol 4,5 bisphosphate

JAE Payne; MR Bleackley; TH Lee; TMA Shafee; IKH Poon; MD Hulett; MI Aguilar; NL Van Der Weerden; MA Anderson

Journal article

The plant defensin NaD1 introduces membrane disorder through a specific interaction with the lipid, phosphatidylinositol 4,5 bisphosphate

JAE Payne, MR Bleackley, TH Lee, TMA Shafee, IKH Poon, MD Hulett, MI Aguilar, NL Van Der Weerden, MA Anderson

Biochimica Et Biophysica Acta Biomembranes | Published : 2016

DOI: 10.1016/j.bbamem.2016.02.016

Abstract

Plant defensins interact with phospholipids in bilayers as part of their cytotoxic activity. Solanaceous class II defensins with the loop 5 sequence pattern "S-[KR]-[ILVQ]-[ILVQ]-[KR]-[KR]" interact with PI(4,5)P2. Here, the prototypical defensin of this class, NaD1, is used to characterise the biophysical interactions between these defensins and phospholipid bilayers. Binding of NaD1 to bilayers containing PI(4,5)P2 occurs rapidly and the interaction is very strong. Dual polarisation interferometry revealed that NaD1 does not dissociate from bilayers containing PI(4,5)P2. Binding of NaD1 to bilayers with or without PI(4,5)P2 induced disorder in the bilayer. However, permeabilisation assays ..

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University of Melbourne Researchers

Marilyn Anderson Author

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Grants

Awarded by Australian Research Council

Funding Acknowledgements

MAA and NLV are funded by the Australian Research Council (DP150104386).MIA acknowledges the support of the National Health and Medical Research Council (#1044327) and the Australian Research Council (DP1110101866).